Why is it common to use absorbance values rather than transmittance values when doing quantitative UV-Vis spectroscopy?

Answers

Answer 1

In quantitative UV-Vis spectroscopy, it is common to use absorbance values rather than transmittance values for various reasons which are given below:


1. Linearity: Absorbance values have a linear relationship with the concentration of the sample according to the Beer-Lambert Law (A = εcl), where A is absorbance, ε is the molar absorptivity, c is the concentration, and l is the path length. This linearity allows for easier determination of unknown concentrations from a calibration curve.

2. Sensitivity: Absorbance values provide greater sensitivity in measurements, especially for low concentrations. As the concentration decreases, the difference between transmittance values becomes less noticeable, making it harder to distinguish between them. Absorbance values, on the other hand, are more distinguishable at lower concentrations, allowing for more accurate analysis.

3. Logarithmic nature: Transmittance values are expressed as percentages, while absorbance values are logarithmic. Logarithmic values are easier to work with in calculations and provide a better representation of the sample's behavior across a wide range of concentrations.



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Related Questions

what is lattice energy and how is it involved in an ionic bond

Answers

Answer:

Lattice energy is an estimate of the bond strength in ionic compounds. It is defined as the heat of formation for ions of opposite charge in the gas phase to combine into an ionic solid.

Explanation:

What are the uses of tritium?​

Answers

Tritium is used as a tracer for biochemical research, animal metabolism studies and ground water transport measurements.

Answer:

biochemical research, animal metabolism studies, and ground water transport measurements.

Explanation:

If you wear contact lenses, why should you remove them while you are in the laboratory?
A)
They can dry out your eyes while you are doing your experiment.
B)
They can hold chemicals that accidentally splash into your eye next to your eye and cause permanent damage.
C)
They can fall out if you do not remove them.
D)
None of the above


Please select the best answer from the choices provided

A
B
C
D

Answers

If a corrosive liquid should splash in the eye, the natural reflex to clamp the eyelids shut makes it very difficult, if not impossible, to remove the contact lens before damage is done.so the answer is B

Contact lenses have adhesive nature

In laboratory perhaps any chemical rushes into your eye then the contact lenses can catch and hold them there

which can cause severe damage to the eye lids

option B is correct

When will the simping end

When will the simping end

Answers

Answer:

Nvr XD

Explanation:

Answer:

the world may never know

Explanation:

what safety equipment do you need if you are working with a strong base

Answers

Answer:

Closed-toe shoes, long pants, a lab coat, safety glasses with side shields or splash goggles, and gloves.

Explanation:

Question 1 of 32
How does soil begin to form?
A. Rock particles clump together in an aggregate.
B. Sediment stays and settles in a location.
C. Bedrock breaks down into smaller particles.
D. Sediment moves to a new location.

Answers

The answer is c I believe! The order that makes the most sense to me is c,d,b,a

write any three difference between shell and nucleus

Answers

Answer:

1)NUCLEUS -> It is the center of an atom which contain protons and neutrons

SHELL-> the space where the probability of finding electrons is maximum in the atom is known as shell

there are mainly four shells KLMN

2)NUCLEUS->The average potential of Nuclei is different from that of an atom.

SHELL -> The average Potential varies.

3)NUCLEUS ->Particles of the shell structure of nuclei are neutrons and protons

SHELL->Particles of the shell structure of an atom are electrons

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PLEASE HELP!!!! ANSWER 2 QUESTIONS FOR 50 POINTS AND BRAINLIEST!

PLEASE HELP!!!! ANSWER 2 QUESTIONS FOR 50 POINTS AND BRAINLIEST!

Answers

1)to see how the food coloring affected each amount of water
2)the amount of water in each cup caused the food coloring to spread either faster or slower

Which of the following statements regarding bond energies is FALSE?

Answers

Answer:where are the statements

Explanation:

\( \huge \color{seagreen} \underline \color{seagreen} \underline \color{seagreen} \tt\star \: QUESTION \: \star\)
0.177 g of a metal produces with dil. HCl 177.0 mL of dry hydrogen gas at 776 mm and 12°C. Calculate the equivalent weight of the metal.

Answers

Answer:

weight of hydrogen÷eq weight of hydrogen=weight of metal÷equivalent weight of metal

yo formula lagayera nikalne

yesma pressure ko kei kaam hunna

Answer:

Hii,kusha

ek problem hó rahi hai mujhe

Radioactive radium has a half-life of approximately 1,599 years. The initial quantity is 13 grams. How much (in grams) remains after 850 years

Answers

The quantity of substance remains after 850 years is 8.98g if the half life of radioactive radium is 1,599 years.

What is half life period?

The time taken by substance to reduce to its half of its initial concentration is called half life period.

We will use the half- life equation N(t)

N e^{(-0.693t) /t½}

Where,

N is the initial sample

t½ is the half life time period of the substance

t2 is the time in years.

N(t) is the reminder quantity after t years .

Given

N = 13g

t = 350 years

t½ = 1599 years

By substituting all the value, we get

N(t) = 13e^(0.693 × 50) / (1599)

= 13e^(- 0.368386)

= 13 × 0.691

= 8.98

Thus, we calculated that the quantity of substance remains after 850 years is 8.98g if the half life of radioactive radium is 1,599 years.

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How many photons of light are emitted when the electrons in 10 hydrogen atoms drop from energy level 5 to 3?


Someone help explain how to do this!!

Answers

When the electrons in 10 hydrogen atoms drop from energy level 5 to 3, a total of 10 photons of light are emitted.

To understand how many photons are emitted, we need to consider the energy levels of hydrogen atoms. The energy levels in hydrogen are quantized, meaning that electrons can only occupy specific energy levels. When an electron transitions from a higher energy level to a lower energy level, it releases energy in the form of a photon of light.

In this case, the electrons in 10 hydrogen atoms are transitioning from energy level 5 to 3. This means that each atom is undergoing one transition. Since we have 10 atoms, the total number of transitions and hence the number of photons emitted is also 10.

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Hi! I need some help with this pleaseeee!!

When two objects of different temperatures are placed in contact with one another, eventually: (choose one option from below)

a) both their average kinetic energy and temperature will be the same

b) their average kinetic energy will be the same

c) neither their average kinetic energy and temperature will be the same

d) their temperature will be the same

Answers

a) eventually they will be in thermal equilibrium

The noble gas electron configuration of S2- isGroup of answer choices[Ar]4s2[Ne]3s2 3p4[Ne]3s2 3p6[Ar][Ne]

Answers

Answer

[Ne] 3s² 3p⁶

Explanation:

Neutral sulfur has an atomic number of 16.

Neutral sulfur has 16 electrons and 16 protons.

However, S²⁻ implies neutral sulfur has gain two more electrons.

So, S²⁻ is negatively charged (anion) and has 18 electrons.

Electronic Configuration

Neutal sulfur (S) → 1s² 2s² 2p⁶ 3s² 3p⁴ → [Ne] 3s² 3p⁴.

Charged sulfur (S²⁻) → 1s² 2s² 2p⁶ 3s² 3p⁶ → [Ne] 3s² 3p⁶.

Therefore, the electron configuration of S²⁻ is [Ne] 3s² 3p⁶.

State the career function of mechanical engineering​

Answers

Answer:

Mechanical engineers design power producing machines, like electric generators, internal combustion engines, and steam and gas turbines, also power using machines, such as refrigeration and air-conditioning systems. Mechanical engineers design other machines inside buildings, such as elevators and escalators.

Explanation:

will magnesium and fluorine atoms most likely form an ionic bond or a covalent bond? 15px

Answers

Magnesium and fluorine atoms will most likely form an ionic bond.

Ionic bonds are formed between elements with a large difference in electronegativity, which is the measure of an atom's ability to attract electrons towards itself. Magnesium and fluorine have a difference in electronegativity of 2.13, which is large enough to form an ionic bond.

In ionic bonds, one atom loses electrons and becomes a positively charged ion (cation), while the other atom gains electrons and becomes a negatively charged ion (anion). In this case, magnesium will lose two electrons to become Mg2+ and fluorine will gain one electron to become F-. These two ions will then attract each other electrostatically to form magnesium fluoride (MgF2), which is an ionic compound.

On the other hand, covalent bonds are formed between elements with a small difference in electronegativity, where atoms share electrons to achieve a stable electron configuration. Magnesium and fluorine have a large electronegativity difference, so they are unlikely to share electrons and form a covalent bond. Therefore, magnesium and fluorine will most likely form an ionic bond.

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Which of the following describes an impact of the specific heat of water on the planet?

Answers

Answer:

A.

Explanation:

specific heat of water is immense as compared to most of other known substances. The result of this characteristic is that water heats slowly and cools slowly. In coastal region and islands, the air above lands gets heated up quickly as compared to air above water.

The impact of the specific heat of water on the planet is that air above the ocean not easily gets heated.

What is specific heat?

Specific heat is define as the amount of heat needed to increase the one unit temperature of one gram of the substance.

Specific heat of water is equal to 1 calorie and it is that much of high as water will require a lot of energy to change its temperature, and not easily gets cool or warm. Due to this water of the oceans almost have the same temperature and air above the ocean not easilt gets warmer as comapre to the air over the coastal area.

Hence, impact of the specific heat of water on the planet explained above.

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calculate the ph of a buffer solution that is 0.329 m in c6h5nh2 (aniline) and 0.108 m in c6h5nh3br.

Answers

The pH of the buffer solution can be calculated using the Henderson-Hasselbalch equation is 4.008. \(pH = pKa + log\frac{[A-]}{[HA]}\).

Where pKa is the dissociation constant of the weak acid (aniline), [A-] is the concentration of the conjugate base (C6H5NH2-), and [HA] is the concentration of the weak acid (aniline, C6H5NH2).
First, we need to determine the pKa value for aniline. This can be found in a reference book or online database and is approximately 4.6.
Next, we can plug in the values we have:
\(\(pH = 4.6 + log\frac{[C6H5NH2-]}{[C6H5NH2]}\)
\(pH = 4.6 + log\frac{0.108}{0.329}\)
Using a calculator, we get:
pH = 4.6 + (-0.592)
pH = 4.008
Therefore, the pH of the buffer solution is approximately 4.008.
We can use the Henderson-Hasselbalch equation to calculate the pH of a buffer solution. This equation takes into account the dissociation constant of the weak acid and the concentrations of the weak acid and its conjugate base. In this specific example, the pH of the buffer solution containing aniline and C6H5NH3Br is approximately 4.008.

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First gets brainliest

First gets brainliest

Answers

Answer:

reactants : before arrow sign

: CH4 and O2

product : after arrow sign

: CO2 and H2O

the answer is the second option

where does energy come from? newsela

Answers

Answer:

All of our energy comes from the sun.

Explanation:

The sun sends out huge amounts of energy through its rays every day. Without the sun, life on earth would not exist, since our planet would be totally frozen.

Where is the blood first filtered?

W
X
Y
Z

Where is the blood first filtered?WXYZ

Answers

Answer:

in w

Explanation:

first blood enter renal artery which enter glomerous( where blood is filtrated) and then to Bowman carpusle(take the filtrated blood)

and then to proximal tubular(absorbed salt) and then to loop of hanle (y)(absorb water) and then to distal tubule then to ureter (z) and then to renal vein(x)(to go to urinary bladder)

The molarity of a solution that contains 28 g KOH (molar mass 56 g/mol) per 250 mL of solution is:

Answers

Molarity = moles of solute/volume of solution in liters

The solute here is KOH. We have the mass of KOH, and we can calculate the number of moles by dividing by the molar mass: (28 g KOH)/(56 g/mol) = 0.5 mol KOH. The volume of our solution is 0.250 L.

So, the molarity of the solution would be (0.5 mol KOH)/(0.250 L) = 2 mol/L KOH or 2 M KOH.

We are ALWAYS conscious (aware) of why we decide to do the things we do:
True or false

Answers

Answer: true

Explanation:

True

Srry I need 20 characters to submit this Answer that’s why I have more words here

Which of these is an oxidation half-reaction?

Answers

Answer:

Fe + 02 - Fe203

Explanation:

Hope this helps

CH3CH2C=CH2)
O=O
?
What is the functional group for this compound

Answers

Answer:

ketone

Explanation:

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PLEASE HELP ME ASAP PLEASE!

PLEASE HELP ME ASAP PLEASE!

Answers

Answer:

the equation is balanced because there are 8 atoms of carbon, 26 atoms of oxygen, and 20 atoms of hydrogen. on each side of the equation.

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Regarding the relationship between equilibrium constants and standard cell potential, which of the following equations is accurate? Select the correct answer below
a. E˚cell = nF/RTln k
b. Delta G = - nF/Ecell
c. E˚cell = (RT/ Nf) ln K
d. E˚cell = 1.0 V/n log K

Answers

Your answer: The accurate equation regarding the relationship between equilibrium constants and standard cell potential is:
c. E˚cell = (RT/nF) ln K

The accurate equation for the relationship between equilibrium constants and standard cell potential is option C: E˚cell = (RT/ Nf) ln K. This equation is derived from the Nernst equation, which relates the standard cell potential (E˚cell) to the equilibrium constant (K) at a specific temperature. The equation shows that the cell potential depends on the temperature, the number of electrons transferred (n), the Faraday constant (F), and the gas constant (R). It also indicates that the standard cell potential is directly proportional to the natural logarithm of the equilibrium constant. Therefore, the accurate equation for the relationship between equilibrium constants and standard cell potential is C.
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This type of chemist understands the structure of living systems and, in turn, their
functions and ways to control them.

Answers

A Biochemist is a type of chemist understands the structure of living systems and, in turn, their functions and ways to control them.

What is the chemistry of living systems called?

The chemistry of living system is known as Biochemistry.

Biochemistry is a study of the chemical changes that occur in living organisms.

Scientists that study biochemistry are called Biochemists.

Biochemistry studies the structure and function of biological molecules such as carbohydrates, lipids, proteins, e.t.c., as well the chemical reaction they undergo.

Biochemistry also studies the energy changes that occur in living systems.

In conclusion, the chemistry of living systems is called Biochemistry.

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What is the mole fraction of KCI in an
aqueous solution that contains
26.3% KCI (weight/weight %)?

Answers

Answer:

The mole fraction is the number of moles of something divided by the number of total moles present. First, let us work out the number of moles of KCl present. A 26.3% w/w solution would contain 26.3 g of KCl per 100 g of solution. Hence, 26.3 g of KCl is 26.374.5513 = 0.353 moles

Explanation:

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61.0 mL of 3.11 M HNO3 were mixed with 68.1 mL of 2.75 M KOH. Find the pOH of the resulting solution

Answers

the pOH would be 1.83 because MaVa=MbVb then calculate
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